CH 461 Study Guide - Final Guide: Catalytic Triad, Competitive Inhibition, Monod-Wyman-Changeux Model

• School: University of Oregon
• Department: Chemistry and Biochemistry
• Course: CH 461
• Professor: Ken Prehoda

Document Summary

For the final exam, also review the study guides for the first two midterms, quizzes, all problem sets, the two midterm exams and each of the practice exams. Understand the differences in structure and function between myoglobin and hemoglobin. Understand the molecular basis for the cooperativity of oxygen binding by hemoglobin, as well as the decreased affinity of hemoglobin for oxygen in the presence of low ph (the bohr effect), and bpg. Understand the experiment in which the proximal histidine was mutated to glycine and imidazole was added. (why it was done and what the result demonstrated). Understand how the mwc model can explain positive cooperativity, and the requirements for cooperativity in mwc. Understand why the mwc cannot explain negative cooperativity. Know how both positive and negative cooperativity arise in this model and how to mathematically relate the equilibrium binding constants to the stability of subunit interfaces. Know how to relate important equilibrium constants using coupled equilibria.