BIS 104- Final Exam Guide - Comprehensive Notes for the exam ( 184 pages long!)

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Break cells to see what happens genetics: essential genes. Redundancy either of 2 genes are enough: genetics tells use what is necessary, but not sufficient. Antibodies specifically recognize single proteins: igg 4 polypeptides (2 identical heavy chain and 2 identical light chain) Western blot: is protein is there or not, how much protein (concentration, how big is the protein. Lyse cell boil cell in sds. Unfold protein and coats with sulfate groups (negative charged) Sds page: little = fast, big = slow. Transfer protein to a membrane nitrocellulose. Immunoprecipitation (ip: method to isolate / purify protein or complex or network from the cell, need antibody against protein of interest, way to isolate antibody : antigen complex. Lyse cell shear force dounce homogenizer. Insoluble proteins and aggregates will go into pellet. Protein a will attach to beads: comes from a bacterium and binds on fc region of igg, pellet = antigen + antibody +beads, supernatant = everything else.

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