CBNS 101 Study Guide - Final Guide: Transmission Electron Microscopy, Scanning Electron Microscope, Electron Microscope
7 Aug 2018
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Microscopy: the ability to see cells and their internal structures is a fundamental requirement of studying cells. Two main types of microscopy: light microscopy & electron microscopy. Light microscopy: incident light is used to illuminate the specimen. Electron microscopy: beam of electrons with a specific wavelength. Resolution: the ability to distinguish between two closely spaced objects. When light (which moves as a wave) passes around the edges of an object at high magnification, light is diffracted, resulting in patterns of constructive and destructive interference. Interference patterns appear as shadows surrounding the object. Lenses on the condenser focus light on the specimen. Contrast in bright-field microscopy is induced by staining. Specimen needs to de-fract light in order to see it. Gfp tagging allows you to look at this in live cells. Gfp tagging allowed researchers to tag specific proteins in live cells! Review questions: transmission, gfp tagging, confocal, transmission, confocal, confocal, antibodies, gfp tagging.
