LIFESCI 3 Final: LIFESCI 3, Final Part 2
Document Summary
Class i rf recognize stop codons, trigger hydrolysis of the peptide chain from the trna in the p site. Class ii rf remove the class i rf from the ribosome. Come in bound to gdp, have a high affinity for class i rf. Exchanges the gdp for gtp, that causes a change in the rf ii shape, causing a lower affinity for class i rf, so class i rf leaves the ribosome. Since class ii rf is gtp bound in the large subunit, it trigger gtp hydrolysis, causing ii rf to leave. Both the rrf and class i rf are mimics of trna. Instead of an acceptor arm, the protein has a group that causes hydrolysis of the acyl linkage. Rrfs help to release the mrna from the ribosome. Rrf translocates, causing uncharged trnas to leave. If3 is recruited and binds to the e site to separate the large and small subunits. Class ii rfs are also g proteins.
