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Midterm

LIFESCI 3 Study Guide - Midterm Guide: Selectable Marker, Genomic Library, ExonucleaseExam


Department
Life Sciences
Course Code
LIFESCI 3
Professor
All
Study Guide
Midterm

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1
LS3 MIDTERM II
INSTR: L. JOHNSON Wed, Nov. 10, 2010
NAME:_________________________________
(last) (first)
I.D. #__________________________________
INSTRUCTIONS
1. This exam is 7 pages long. Page Value Score
Before starting make sure
you have all the pages. 2 23
2. WRITE YOUR NAME ON EVERY PAGE. 3 16
3. No credit will be given for an 4 20
illegible answer.
5 20
4. WRITE ONLY IN THE SPACE PROVIDED.
NO OTHER ANSWER WILL BE GRADED. 6 21
Total 100
KEY

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2
NAME:_________________________________ ID __________________________
(last) (first)
(17 pts) 1. In prokaryotes the start sites for transcription and translation are distinct.
a) What determines the site of transcription initiation? (2 pts)
The position of the promoter sequence.
b) What are the regions called that are transcribed but not translated? (2 pts)
5’ UTR and 3’ UTR (or untranslated regions)
c) What determines whether an AUG will be used to initiate translation? (2 pts)
An AUG will be used for inititiation only when it has a Shine-Delgarno (or
RBS-ribosome binding site) positioned correctly upstream
d) Does +1 refer to the start of the open reading frame or the start of
transcription? (2 pts)
Start of transcription
e) Name any initiation factors necessary for transcription initiation. (2 pts)
Sigma factor
f) Name any initiation factors necessary for translation initiation. (3 pts)
IF1, IF2 and IF3
g) What are the substrates for RNA synthesis? (2 pts)
(ribo)nucleoside triphosphates or NTPs or rNTPs or ATP,CTP, TTP and GTP
h) What are the substrates for protein synthesis? (2 pts)
Amino-acyl tRNAs or charged tRNAs
(6 pts) 2. Suppose RNA synthesis occurred in 3’5’ and strands are antiparallel in both
DNA and DNA/RNA hybrids. Label the figure below accordingly.
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