LIFESCI 3 Study Guide - Midterm Guide: Selectable Marker, Genomic Library, ExonucleaseExam
Course CodeLIFESCI 3
This preview shows pages 1-2. to view the full 6 pages of the document.
LS3 MIDTERM II
INSTR: L. JOHNSON Wed, Nov. 10, 2010
1. This exam is 7 pages long. Page Value Score
Before starting make sure
you have all the pages. 2 23
2. WRITE YOUR NAME ON EVERY PAGE. 3 16
3. No credit will be given for an 4 20
4. WRITE ONLY IN THE SPACE PROVIDED.
NO OTHER ANSWER WILL BE GRADED. 6 21
Only pages 1-2 are available for preview. Some parts have been intentionally blurred.
NAME:_________________________________ ID __________________________
(17 pts) 1. In prokaryotes the start sites for transcription and translation are distinct.
a) What determines the site of transcription initiation? (2 pts)
The position of the promoter sequence.
b) What are the regions called that are transcribed but not translated? (2 pts)
5’ UTR and 3’ UTR (or untranslated regions)
c) What determines whether an AUG will be used to initiate translation? (2 pts)
An AUG will be used for inititiation only when it has a Shine-Delgarno (or
RBS-ribosome binding site) positioned correctly upstream
d) Does +1 refer to the start of the open reading frame or the start of
transcription? (2 pts)
Start of transcription
e) Name any initiation factors necessary for transcription initiation. (2 pts)
f) Name any initiation factors necessary for translation initiation. (3 pts)
IF1, IF2 and IF3
g) What are the substrates for RNA synthesis? (2 pts)
(ribo)nucleoside triphosphates or NTPs or rNTPs or ATP,CTP, TTP and GTP
h) What are the substrates for protein synthesis? (2 pts)
Amino-acyl tRNAs or charged tRNAs
(6 pts) 2. Suppose RNA synthesis occurred in 3’5’ and strands are antiparallel in both
DNA and DNA/RNA hybrids. Label the figure below accordingly.
You're Reading a Preview
Unlock to view full version