MCD BIO 165A Study Guide - Midterm Guide: Receptor Tyrosine Kinase, Epidermal Growth Factor Receptor, Signal Transduction

• School: UCLA
• Department: Molecular, Cell, and Developmental Biology
• Course: MCD BIO 165A
• Professor: All

Document Summary

-is this protein functionally important for my pathway? sirna, crispr. -does a signal change the abundance of components in the signaling pathway? real time pcr, western blotting. -does a signal change the localization of components in the signal transduction pathway? immunofluorescence, fusion proteins. Signaling pathways often involve changes in the phosphorylation states of proteins. Binding of ligand, induces dimerization of the two inactive receptor subunits. Formation of a dimer leads to phosphorylation and further intracellular signaling events. Structure of egf receptor: inactive monomer and active dimer. Activation of a receptor tyrosine kinase leads to activation of the small intracellular signaling molecule: In the absence of ligand, ras is bound to gdp and receptors are present as monomers. Ligand binding induces dimerization and cross-phosphorylation; activation of ras is mediated by grb2- Sos which induces the exchange of gtp for gdp on ras.