MIMG 101 Study Guide - Final Guide: Transmission Electron Microscopy, Fluorescence Microscope, Cellulosome

• School: UCLA
• Department: Microbiology, Immunology, and Molecular Genetics
• Course: MIMG 101
• Professor: G U N S A L U S& B R A D L E Y

Document Summary

Small amounts of light are difracted by the specimen. Anything above 40x resoluion from objecive requires oil. Need enough light to gather and hit specimen. The oil allows enough light to gather and focus onto the specimen. Don"t need to know much about light source for this one. Direct: atached to anibody itself, atached to a speciic anigen. Indirect: primary anibody binds anigen, and secondary ataches to primary anibody, carrying luorescent tag. Ampliicaion of the signal, because muliple secondary anibodies can bind the primary anibody. Advantage: tagging speciic proteins, rather than simply looking at the sample like with other types of microscopy. Say you"re looking at a protein and you don"t it"s a membrane protein. Now you can see where it is localized. Super resoluion: overcomes limitaion of resoluion of light, beyond resoluion of . 2 micrometers. The second light source is canceling out certain wv of the irst pulse. Looks more like original structure rather than blurry line.