MIMG 101 Study Guide - Quiz Guide: Leucine, Threonine, Glutamine

• School: UCLA
• Department: Microbiology, Immunology, and Molecular Genetics
• Course: MIMG 101
• Professor: G U N S A L U S& B R A D L E Y

Document Summary

Exercise #3 reply template: blast and genome comparison. Blastp uses and a protein query and protein database while blastn uses a nucleoide query and nucleoide database. Blastn is less exact because of the wobble posiion, which allows for variable pairing of the trna with mrna. Thus, nucleoides are more diicult to accurately blast. An amino acid sequence is less ambiguous. b. the top hit (prolyl-trna synthetase) has a score of 1126, e-value of 0. 0, and % idenity of 100%. The protein serves to interpret the mrna geneic sequence by covalently linking amino acids to their speciic trna molecules. The protein also paricipates in ediing mischarged trnas: the top hit, prolyl-trna synthetase, has a score of 1118, e-value of 0. 0, and % idenity of 99%. I think this protein is indeed found in this strain because the e-value is 0, meaning the probability that this match occurred by chance is very low to none.