MIMG 102 Study Guide - Midterm Guide: Virus Quantification, Filter Paper, Antibody

• School: UCLA
• Department: Microbiology, Immunology, and Molecular Genetics
• Course: MIMG 102
• Professor: Dasgupta Asim

Document Summary

Isolating host range mutants: initial plaque assay on host range cells, so both wt & host range virus can grow: when measuring rna pol activities: measure transcription. Take dna template of any sequence with certain promoter (e coli/class i or class ii/iii) Give template different fractions, see # of peaks, give hot utp and other normal ntp. 1-step growth curve for virus: take out aliquot at time points, separate into 2 parts, run sds-page, western for proteins (1 for each orf) If asked to determine if dna/rna synthesis follows growth curve: take out aliquots & do southern/northern blot. If using probe: northern or southern: never pulse in main solution! (all protein labeled); add pulse in aliquots of different time points. M13 doesn"t kill cells -> can be kept frozen & used for next time. T7 lyses cell -> every time need to clone has to start from scratch.