MIMG 168 Study Guide - Final Guide: Homologous Recombination, Duffy Antigen System, Ku80

• School: UCLA
• Department: Microbiology, Immunology, and Molecular Genetics
• Course: MIMG 168
• Professor: P.J. Johnson

Document Summary

If ko, then no nhej and homologous recombination is used. Want to bump up chances of doing homologous recombination from 5%. Homologous recombination is targeted transfection and more conserved. When using a plasmid or other transfection, you"re not sure if the parasite takes it, so you use a drug marker with a drug resistance gene, the parasite won"t die. Could ko something essential, but won"t be important: falcii is more deadly. Jumps from humans to gorillas to humans and has no way to keep the host alive. Causes knobs to form on the rbc which allows for adherence which causes blockages and cereberal malaria. Both have rings, but falcium has more rings than vivax. If you have duffy antigen, more resistant to p. falci. Glides around in the cell until it finds the host cell. Uses micronemes to attach to the host cell.