DGS 3223 Study Guide - Midterm Guide: Ribosomal Rna, Thymidine, Interphase
8 Sep 2016
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Steps of sequential staining: photograph, remove coverslip and all oil, stain, destain/airdry slides after each procedure, keep fluorescent excitation to minimum, make extra slides. Nor requires fresh slides or slides with no trypsinization. Mounting media must have a refractive index of below 1. 52, closest to 1 as possible. Permanent stained slides must be kept for 3 years: no guideline for fluorescent stained slides. Cell synch/chemical elongation: uses propase/prometaphase chromosomes (longer, cell synch. After 17 hours, cells released from block with addition of lots of thymidine. Works at different point in cell cycle: chemical elongation. Eb is added to culture 2 hours before harvest. Cra was created bc eb is so dangerous (chromosome resolution additive) 4 traditional special stains most frequently used: c-banding, nor, dapi/da, q-banding. Steps of fish: denature double-stranded dna, introduce probe, hybridize probe to sequence of single-stranded dna, complete dna synth, detect fluorescence. Alpha satellite probes: 171 bps, used for. Detection of aneuploidies in interphase & metaphase cells.
