NURS 3110- Final Exam Guide - Comprehensive Notes for the exam ( 180 pages long!)
Document Summary
Objectives: differentiate between optical methods for visualizing cells and cell structures, distinguish between eukaryotic and prokaryotic cellular structures and function of each, describe staining techniques in the identification of gram positive vs. gram negative organisms. Identify differences between prokaryotic gram negative and gram positive cellular envelopes. Staining: the gram stain, important role in taxonomic characteristics of bacteria, basis of different reaction is cell wall structure, procedure, application of basic dye, crystal violet, cell walls treated with alcohol; Iodine solution applied; all bacteria now stained blue: gram + cells retain crystal violet-iodine, remain blue, gram - cells are completely decolorized, red dye safranin applied so gm - cells take contrasting color. Identify the array of conditions that impact the microbial proliferation. Culturing microorganisms: types of media, transport media (culturettes, basal media (supplies minimal (base) growth requirements, also called general purpose, enriched media: basal & additives to enhance growth.
