MCDB 101A Study Guide - Midterm Guide: Auxotrophy, Writhe, Dna Supercoil
27 Feb 2015
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Understand when to apply the rule of two in determining recombination frequencies/map. Understand how trans test results are affected by the dominant mutations and alpha complementation and how the former can be detected with a dominance test. Understand the major points of the watson-crick paper (see watson & crick questions in study distances in a cis test. Know the three chemical components of a nucleotide and which bases are purines and pyrimidines; know structural difference between a deoxyribonucleotide and ribonucleotide. Know which base pairs with which in double-stranded dna and the number of hydrogen bonds. Know chargaff"s rules and how to use them in determining if a dna molecule is single or. Know the difference between positive and negative supercoiling and the relationship between formed between base pairs double-stranded linking number, twist , and writhe. Experiments: know what information was provided by franklin"s x-ray diffraction of dna.
