BIMM 101 Study Guide - Final Guide: 2Degrees, Restriction Enzyme, Plasmid
Document Summary
Serial remember to start with starting concentration and final concentration. Ex) given . 75g/ml = 750000 g/ml stock and 5ml of 1 g/ml end. No dilutions greater than 1:100, give set up. Pros + cons of image studio lite. Properties of dna and effects on dna. Gene of interest (insert) e. g. gfp/mcs (multicloning site) where all restriction enzymes are. Try to choose at start of dna sequence. Check to see if certain experiment has correct amount of bands. If not, redesign primers, purify sample, change temperature, concentration, etc. Dna repair through non-homologous end joining (nhej) generally results in deletion. Kinase - adds 5" phosphate to end of pcr for ligation. Ligase - connects 5" and 3" ends of pcr product. Dpni - restriction enzyme that cuts methylated dna to break down template but not plasmid for transformation. Https://quizlet. com/277347720/bimm-101-finals-studying-flash-cards/ has some terminology from lab/lectures that might be relevant. Https://quizlet. com/246277424/bimm-101-recombinant-dna-techniques-flash-cards/ mass review of techniques and terms used.
