BIBC 100 Study Guide - Midterm Guide: Ramachandran Plot, Steric Effects, Helix Bundle
Document Summary
4 levels of protein structure: 1: aa residues, 2: alpha helices, beta strands, 3: polypeptide chains, 4: assembled subunits. Hydrophobic residues in helices interact to stabilize structure to hold calcium binding residues in place: ex of ef hand use: calcium-dependent protein kinases (cdpks) activated by conformational change after calcium binds to ef hand motifs. Inactive state when no calcium present and helices block kinase domain. When calcium binds, helices conformation changes and kinase domain exposed to phosphorylate other proteins for signal transduction: plasmodium falciparum cdpk: antimalarial drug target, malaria caused by protozoan that proliferates within blood cells to complete its life cycle. To invade blood cells, p falciparum must form a microneme (penetrates red blood cells to initiate invasion) and pfcdpk1 is essential to this process. Pfcdpk1 is a target for highly sought after anti-malarial drugs: left-handed loose helix, ex: collagen is loose left-handed triple helix.
