MCB 252 Study Guide - Final Guide: Neurofibrillary Tangle, Spindle Apparatus, Basal Body
3 Sep 2018
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Document Summary
Plus end extends out and minus in the basal body. Assembled from heterodimers of alpha and beta tubulin (these are 40% identical) Dimers are stable and rarely dissociate ( tubulin = dimer). Tubulins are highly conserved (>75% identical between plants and animals, alpha and alpha and beta and beta) Alpha - binds gtp but does not hydrolyze it and it is non-exchangeable. Beta - binds gtp but hydrolyzes it and can exchange it. There is a seam where alpha and beta are adjacent to each other. Plus ends grow faster than minus ends. Gtp tubulin added to + end and then exchanged to gdp. Gtp monomers are only at the + and were recently added. Lower critical concentration at + end and higher at - end. Cilia (short, many copies), flagella (long, one copy), mitotic spindle. Can more liquid over surface or move itself through liquid. Plus ends out and minus ends in.
