BIOLOGY 172 Study Guide - Midterm Guide: Taq Polymerase, Polymerase Chain Reaction, Dna Polymerase
Document Summary
Words: dideoxynucleotides, sanger sequencing, shotgun sequencing, genome annotation, transposable elements, orf- open reading frame. Amplifies pieces of dna (millions copies of dna sequence in few hours) If you want multiple copies of gene not whole genome in test tube. Useful if you need multiple samples for testing . Separate strands of sna using heat in replication in cell helicase does this. Instead in pcr we use heat to separate dna denature. Two primers anneal to complementary sequence on dna template. Need to design primers so anneal at begininning of template strands. Complementary to template strand which is 5" 3" so primer = 3" 5". One primer for each (top and bottom) strand. Primer form hydrogen bond with template strands. Primer ending with 3" end can add nucleotides: extension. Dna polymerase synthesizes new dna strand by extending primers (adding to. Dna synthesis takes place in 5" 3". Each round doubles number of molecules 2n.
