BIOCHEM 524 Study Guide - Quiz Guide: Reaction Rate, Porphyrin, Cooperative Binding
11 Apr 2014
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Here are some guidelines as to what i would like you to know for quiz 2. I"ve been a little more explicit than for quiz 1, hence the longer list. Heme: nature of coordination bonds between fe and porphyrin nitrogens: has 6 possible coordination bonds. 4 n atoms of porphyrin ring coordinate bond with heme. Oxygen binding curve: hyperbolic: as o2 concentration increases more and more o2 molecules bind to the heme groups until at very high o2 concentrations virtually all heme groups have been bound. Changes in mb structure upon oxygen binding: a his residue on the e7 alpha-helix forms a h-bond w/ o2 when it binds, hemoglobin (heterotetramer with two different chains; red blood cells) Cooperative binding of oxygens to the four subunits: the 4th o2 taken up by hemoglobin binds with about 100x greater affinity than the first.
