MATH 509 Midterm: MATH 509 USC 509S07Fs
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The strategy should include more than one approach to demonstrate. Questions 1-6 refer to this situation: you are able to partially purify an enzyme activity from the membrane fraction of the bacterium, thermotoga maritima. Silver stained sds-page shows three distinct bands that have co-purified but you do not know which protein band(s) correspond(s) to your enzyme: (10 pts) give a strategy for identifying the complete primary structure of the unknown protein. Provide the names or descriptions of the biochemical manipulations and the analytical tools you would use to identify the protein. Specific names of the tools are not necessary but state their purpose and what information they provide. Give the expected results from each step and potential problems. convincingly that a particular protein band is responsible for the deoxyribonucleotide phospho-hydrolase activity. known. You may assume that the complete thermotoga maritima genomic sequence is. Bims 503 exam i: (5 points).
