BIOL 2030 Study Guide - Midterm Guide: Thermus Aquaticus, Ethidium Bromide, Mouthwash
29 Aug 2016
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Gel electrophoresis: to separate dna,rna,protein by size, gel made of agarose, starch, acrylamide to make 3d thing of fibers. Loading buffer:glycerol (to increase density) and tracking dye (ahead of dna mole. s to see how far our smallest mole. s have traveled) Dye: ethidium bromide, inserts itself between bases in double stranded dna. It"s a mutagen so need gloves and handle it with care. Genomic dna- all the dna found in a cell or organism. Instagene- has negatively charged chelex beads to bind to divalent cations (mg) that help enzymes degrade dna template. If it is left in dna mixture its bad: the same divalent cations are used to help taq polymerase (from. Thermus aquaticus bacteria living in hot springs, able to withstand denaturing temps so optimal, it amplifies short dna segments well) Cheek cells boiled and lysed- cations bound to beads and heat inactivates dna degrading enzymes. 1-2ml of mouthwash: too much dna will saturate binding capacity of instagene-> then centrifuge.
