BIOL 4320 Final: Final Review
16 Jun 2016
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Document Summary
Aerotolerance: commonly assessed by growth in fluid thioglcollate. This medium will be sterilized before inoculation, typically by using an autoclave. During autoclaving, molecular oxygen levels drop and many organisms who need oxygen can"t live. Obligate/strict can only grow under anaerobic conditions. Facultative: can growth in the presence of absence of oxygen. Aerotolerant: doesn"t need oxygen so it grows fine down on the bottom. Aerolterant means they can tolerate toxic oxygen species formed by o2: aerobe. Broth is good when you want a lot of cells. A plate gives you a lot of surface area so you can isolate one bacterium from another/colonies. Aseptically: without contamination of yourself, other, the environment, the source culture, or the medium being inoculated. Media: broths: used to grow microbes when fresh cultures or large numbers of cells are required. Differential media broths are used in microbial identification: agar slants: used to grow stock cultures that can be refrigerated after incubation and maintained for several weeks.
