BIOL 1105 Study Guide - Midterm Guide: Single-Nucleotide Polymorphism, Noncoding Dna, Somatic Cell Nuclear Transfer
21 Mar 2017
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Lesson 19: genomics: distinguish between a genetic map and a physical map. Genetic map: provide relative locations of genes, determined by recombination frequency, first knowledge about genomes came from constructing genetic maps. Physical map: shows actual location of landmarks, restriction maps are a common type, dna is cut with restricting enzymes and the banding pattern is analyzed to generate a map, distance is measured in bp or kb. 1000 bp = 1 kb: chromosome banding and fluorescence in situ hybridization (fish) Fish: dna probes with fluorescent labels hybridize at the site of a specific gene on the chromosome. Large scale physical map low resolution: sequence tagged sites. Small stretches on dna that are unique to the genome of a species and only occur once. Sts sites are defined by pcr primers. Pcr can be used to detect the presence of a particular sts. Fragments of dna can be pieced together by analyzing overlapping.
