BIOL 1105 Lecture Notes - Lecture 19: Alternative Splicing, Uniprot, Synthetic Biology
13 Nov 2017
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Document Summary
Distinguish between a genetic map and a physical map. Provide relative locations of genes based on recombinant frequency. First knowledge of genomes came from this. Shows the actual location based off of dna landmarks. Dna is cut with restriction enzymes and the banding pattern is analyzed to create a map. Distances measured in base pairs or kilobases (kb) Fluorescence in situ hybridization (fish) - dna probes with fluorescent labels hybridize at the site of a specific gene on the chromosome. Small stretches of dna that are unique to the genome of a species and only occur once. Defined by pcr primers = easily detected by pcr. Four reactions are set up, one for each base. Dideoxynucleotides used to prevent further nucleotides from being added. Sequence can be determined by reading the nucleotides in order of. Can sequence 50,000 bp in a few hours. Dideoxynucleotides have fluorescent labels with use 4 different colors.
