BIO 3110 Study Guide - Final Guide: Cytochrome C Oxidase, Hydrogen Sulfide, Bromothymol Blue
Document Summary
Hydrogen sulfide production = black: types of fermentation. Organisms that ferment these sugars produce acid in the butt and on the slant, producing a large amount of acid, turning entire medium yellow. Organisms that ferment glucose produce less acid since glucose is in limited supply. While there is enough acid produced to initially turn the entire medium yellow, after 24 hours the glucose gets used up. The organisms continue to grow only on the surface of the slant, where it could oxidize peptone, producing ammonia. Inability to ferment any of the sugars, using peptone instead for energy. Use peptone as an energy source, oxidizing it to ammonia. This organism might be a strict aerobe, only growing on the surface of the slant: results. Fermentation of lactose and sucrose a/a at 24 and 48 hrs. Glucose fermentation a/a at 24 hrs, k/a at 48 hrs. Peptone utilization (cannot ferment any of the sugars) k/no change at 24 hrs,
