Biology And Biomedical Sciences BIOL 2960 Study Guide - Quiz Guide: Clustal, Sodium Hydroxide, Cloning
14 Aug 2018
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Transcriptional regulation of the lac operon: operon: a group of genes that are transcribed in a single polycistronic mrna molecule. Is very similar to lactose except permease is not necessary for it to enter the cell. I-: blue: transcription is on, meaning there is beta-galactosidase that will, 3: x-gal + iptg break down x-gal, wildtype: blue. Iptg acts as an inducer to the repressor, x-gal is broken down by beta-galactosidase: y-: blue. Iptg does not need permease to enter the cell, transcription occurs: z: white, x-gal is not broken down due to the mutated beta-galactosidase. I-: blue: transcription is on, meaning there is beta-galactosidase that will break down x-gal. In pcr mix: template dna, 2 primers, taq polymerase, dntp"s, buffer + mgcl2. Cycle-sequencing of the 16s rrna gene pcr product. If the vector does not have an insert, then it should not be able to re-ligate as the plasmid already cut it and it has been treated by phosphatase.
