BIOL 112 Chapter Notes - Chapter Unit 2 & 3 - Final Exam Content: Purine, Genetic Engineering, Adenine
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BIOL 112 Full Course Notes
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Unit -2 dna replication in vitro-polymerase chain reaction. The polymerase chain reaction selectively amplifies regions of dna: dna that exists in the nuclei of your cells is present in two copies per cell, except for. Because the pcr reaction is essentially a dna synthesis reaction, it requires the same basic components used by the cell to replicate its dna. 1: two primers - two short sequences of single-stranded dna are required for the dna polymerase to start synthesis. Enough primer is added so that the number of primer. Dna molecules is much greater than the number of template dna molecules: the primer sequences are oligonucleotides (oligos is the greek word for few ) produced by chemical synthesis and are typically 20 30 nucleotides long. 3: gel electrophoresis can separate dna fragments produced by any means, not only. Genomic dna can be cut with certain enzymes and the resulting fragments separated by gel electrophoresis, as described in the next section.
