Myosin type V is a two-headedmyosin that operates as a transport motor to move its attachedcargo along actin filaments. Its mechanism is similar to that ofmuscle myosin, but its acts positvely, like kinesin. The reactiioncycle diagrammed here (ABOVE PICTURE) begins with both myosin Vheads bound to an actin filament. ADP is bound to the leading head,and the nucleotide-binding site in the trailing head isempty.
(a) Based on your knowledge ofthe muscle myosin reaction cycle, propose a reaction mechanism formyosin V, starting with the entry of ATP.
(b) How does each step of theATP hydrolysis reaction correspond to a conformational change inmyosin V?
Myosin type V is a two-headed myosin that operates as a transport that operates as a transport motor to move its attached cargo along actin filaments. Its mechanism is similar to that of muscle myosin, but it acts processively, like kinesin. The reaction cycle diagrammed here begins with both myosin V heads bound to an action filament. ADP is bound to the leading head, and the nucleotide-binding site in the trailing head is empty. Based on your knowledge of the muscle myosin reaction cycle (Fig. 5.35), propose a reaction mechanism for myosin V, starting with the entry of ATP. How does each step of the ATP hydrolysis reaction correspond to a conformational change in myosin V?
Show transcribed image text Myosin type V is a two-headed myosin that operates as a transport that operates as a transport motor to move its attached cargo along actin filaments. Its mechanism is similar to that of muscle myosin, but it acts processively, like kinesin. The reaction cycle diagrammed here begins with both myosin V heads bound to an action filament. ADP is bound to the leading head, and the nucleotide-binding site in the trailing head is empty. Based on your knowledge of the muscle myosin reaction cycle (Fig. 5.35), propose a reaction mechanism for myosin V, starting with the entry of ATP. How does each step of the ATP hydrolysis reaction correspond to a conformational change in myosin V?