BIO373H5 Chapter Notes - Chapter 6.6: Fluorescent Tag, Shotgun Sequencing, Temperature Gradient Gel Electrophoresis
Document Summary
In lab samples, measurements reveal what it might be not what is actually happening in field sites. Lab practices establish control for us and are reductionist but these also contribute to heisenberg"s principle. In situ process is affected by the natural microbial communities made of cells. The luxury of the lab"s conditions cause process to differ. Mircoroganisms away from home can be looked at with microscope. But little is known about the 3d structure of their environment. Poor design of lab conditions lead to low cultivation, but some organisms are just unculturable. + biomarkers are used, it"s a challenge to verify accuracy of results. Pflas have traits like carbon atoms, number of double bonds and stereochemistry. The sample needs to be handled well to not get contamination and should be frozen to avoid changes. Nucleic acid extraction isolates nucleic acids from debris + solids through lysis.
