Biology 1002B Chapter Notes - Chapter Outcomes: Nucleic Acid Hybridization, Reactive Oxygen Species, Nuclear Membrane
2 May 2019
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Origin of endomembrane system, nuclear membrane, er etc. Single-stranded dna (gene interested) is labeled with a probe. Through complementary base pairing it will bind to single-stranded dna. Will hybridize the copy of the gene on the gel. The gene cpn60 and the lack of mitochondria in some eukaryotic cells. Cpn60: chaperone helps imported proteins fold correctly -> coming form the cytosol. But has this gene has no function in cells without mitochondria. Reasons why you would move genes from organelles to nucleus. The environment can be toxic and damage dna. Produces reactive oxygen -> oxidizes dna (steals e-) -> damages dna. Save energy in have two copies in nucleus. Nuclear genes expression offers many more options for generating products. Relative sizes of typical mitochondrial, chloroplast and nuclear genomes. Chloroplast genome are reduced relative to the nuclear genome. Explanation for why a genome might have more genes than the total number of proteins coded.
