BIOL-242 Chapter Notes - Chapter 17: Hematocrit, Megakaryocyte, Polycythemia
28 Apr 2020
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To prepare plasma, you need anticoagulants to keep the blood liquid. After putting it in the centrifuge, the plasma will be in the top part. To prepare serum, you do not need coagulants, don"t need to add anything. Just put blood in, then put it in the centrifuge. Serum will be at the top coagulate by itself) Serum has less substances floating on the surface. A hematocrit determines the volume of rbcs described as the percentage of rbcs in a whole blood sample. Tube of blood is centrifuged to pack the red cells at the outer end of the tube, separating them from the wbcs, platelets, and plasma. After measuring length of rbc column and the total length of the blood column, percentage of rbcs can be calculated. Abnormally high hematocrit: 65%+ indicative of polycythemia. Hematocrit with rbcs below normal level: type of anemia.
