CAS BI 325 Chapter Notes - Chapter 6: Muscarinic Agonist, In Situ Hybridization, Acetylcholine Receptor
2 Jan 2018
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Immunochemistry : -find molecule, chemically purify (elicit response by coupling to larger molecule) In situ hybridization:localizing specific mrna transcripts for proteins. In vitro studying: bathed in solution of high k+ concentration which causes large membrane depolarization. Clarification: glutamate will activate any of these receptors, but ampa (mimicker) only has response @ its receptorect. Opioids = broad class of opiate-like chemicals (natural & synthetic) Endorphins - naturally occurring nt that act on opioid receptors. Describes using technique of radioactively or non radioactively labeled. -many peptide containing-neurons violate this principle: usually amino acid or amine + peptide. -choline is taken up by cholinergic axon terminals w cotransport of na+to power the choline movement. Rate-limiting step : availability of choline limits how much ach can be synthed in axon terminal. Ache opposed to chat) bc also manufactured by . Tyrosine hydroxylase (th) : catalyzes first step in catecholamine synthesis. All contain enzyme (conversion of tyrosine to .
