BIO* - Biology BIO* M121 Chapter Notes - Chapter 1: X-Ray Crystallography, The Double Helix, Nitrogenous Base

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Nucleotide = phosphate group, nitrogenous base, and pentose sugar. Phosphate group is bond to 5" carbon of sugar. Nitrogenous base is bonded to 1" carbon of sugar. A nucleoside is the nitrogenous base + pentose sugar. Nucleic acid primary structure is the order of the nucleotides. The strands have directionality a 3" end and 5" end. Nucleotides are added to the 3" end of the chain (e. g. they polymerize in a 5" 3" direction) Sequences are also typically written this way. The addition of phosphate groups generates strong repulsive forces, raising the potential energy of the substrate molecules. This makes an otherwise nonspontaneous reaction possible. The potential energy stored in activated nucleotides is release when the pyrophosphate is removed. Atp is an example of an activated nucleotide. Energy is released when phosphates are removed by hydrolysis. The potential energy in atp is used to drive many other cellular process. Atp is the major energy currency molecule in the cell.

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