BOT 4734C Chapter Notes - Chapter 1: Agrobacterium Tumefaciens, Botany, Transgene
Document Summary
***agrobacterium-mediated transformation revolutionized plant science research, plant biotechnology*** Dna transfer is a relatively (cid:862)rare(cid:863) event. Only very few cells get dna integration initially: need to eliminate untransformed cells. Cultivate transformed cells to grow and develop. Only under selectable condition: selection genes/conditions. Addition of antibiotics (example: kanamycin) or other compounds toxic to untransformed cells. Transformed cells are able to detoxify the selection reagent. Selection genes are always closely located near the gene of interest (target gene) in the transgene vector. Selection gene and target gene are closely linked. (cid:862)if the o(cid:374)ly tool you have is a ha(cid:373)(cid:373)er, the(cid:374) everythi(cid:374)g looks like a (cid:374)ail(cid:863) A circular dna often found in bacteria. Engineered for different purposes: gene modification, amplification, verification. **a ;plasmid that can be easily introduced to bacterial host for multiplication** Plasmid dna can be easily introduced into bacterial or fungal cells.
