BOT 4734C Chapter Notes - Chapter 3: Real-Time Polymerase Chain Reaction, Amplicon, Housekeeping Gene
Document Summary
How to use pcr to identify transgenic plants: extract dna and do pcr: Transgenic dna plus primers 1 & 2. Wt dna plus primers 1 & 2. 30 cycles of pcr, run samples on gel. Real-time pcr & quantitative pcr (qpcr: pcr is a technology in molecular biology used to amplify a single or a few copies of a piece of dna across several orders of magnitude. Generating thousands to millions of copies of a particular dna sequence. Real-time pcr: technology to sensitively detect the real-time pcr amplification of nucleic acid targets. Similarities in real-time pcr and traditional pcr: reagents: Dntps, pcr buffer, taw polymerase: cycling conditions: Differences in real time pcr and traditional pcr: traditional: Visualization on gel: real time pcr: Fluorescence is added to each reaction prior to detection. How does qpcr work: after each cycle, the amount of target dna in the reaction tube theoretically.
