BIOL 2344 Chapter Notes - Chapter 21: Antigen, Western Blot, Restriction Enzyme
23 Oct 2017
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21. 1 gene cloning using vectors: gene cloning ~ production of many copies of gene using molecular methods, such as. Pcr or introduction of gene into vector that replicates in host cell. Cloning experiments may involve two kinds of dna molecules: chromosomal dna and. Restriction enzymes cut dna into pieces and dna ligase joins the pieces together: restriction endonuclease (enzymes) ~ endonuclease that cleaves dna restriction enzymes used in cloning experiments bind to specific base sequences and then cleave. Gene cloning involves the insertion of dna fragments into vectors, which are propagated in. Dna library may be constructed using genomic dna or cdna: dna library ~ collection of many hybrid vectors, each carrying particular fragment of. Dna from larger source: genomic library ~ dna library of recombinant vectors that carry chromosomal dna fragments, cdna library ~ dna library whose recombinant vectors carry cdna inserts.
