BIOL 2051 Chapter Notes - Chapter 2: Fluorescence, Crystal Violet, Oil Immersion
Document Summary
In library on first floor at reserve desk: Microbiology: an evolving science- call number is rx52. Size of prokaryotes: prokaryotes are generally smaller than eukaryotes, small size allows nutrients to reach all parts of cell quickly, prokaryotic cells can be as small as 0. 2 m. The smallest eukaryotic cells are 2 m: cocci- sphere shaped bacteria- typically have diameter of 2 m, some microbiologists have proposed that bacteria smaller than 0. 2 m exist in nature, cells referred to as nanobacteria. Microscopy: simple microscopes, one lens, magnify 50 300x, compound microscopes, multiple lenses, much higher magnification. Ones in 2051 lab magnify up to 1000x. Resolution ability to distinguish between 2 objects that are close together. Correct each other"s aberrations (imperfections: use shorter wavelength of light. Blue light (~400nm) is shortest wavelength so gives highest resolution. Increase numerical aperture (measure of light gathering ability of objective lens) Use wider lens that is closer to specimen.
