BIOL 4246 Chapter : 6 8 12 Elongation And Termination
Document Summary
To have this activation energy, rna polymerase transcribes from the promoter away from oric creating transient positive supertwisting in front and compensatory negative supertwisting. As soon as rna poly leaves they cancel each other out again. Combination of dna bending, negative twist, allows dnaa to separate the strands at the at bp rich directories. Use a ssb to trap the ssdna as ssdna. Allows dnac to assemble dnab around the dna (6 subunits). will go 5" to 3" until it reaches the fork then it will separate the ds strands it meets. Eventually it covers a primase site, dnag is primase and will make an rna primer from which dna poly. Tau subunit dimerizes it with another rna poly iii at the fork. Tau binds dnab and increases rate of strand separation. Kicks helicase into gear and they go 1000 nt/sec.
