BIOL 1111 Chapter Notes - Chapter 4.1: Electron Microscope, Micrograph, Scanning Electron Microscope
30 Oct 2017
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Document Summary
Microscope - a magnification tool that enables researchers to visualize the structure and inner workings of cells. Micrograph - an image taken with the aid of a microscope. 3 important parameters in microscopy are resolution, contrast, and magnification. Resolution - a measure of the clarity of an image. Contrast - relative differences in the lightness, darkness, or color between adjacent regions in a sample. Magnification - the ratio between the size of an image produced by a. Microscopes are categorized into 2 groups based on the source of illumination microscope and its actual size. Light microscope - utilizes light for illumination. Electron microscope - uses a beam of electrons for illumination. Resolution is improved when the illumination source has a shorter wavelength. The wavelength of an electron beam is much shorter than visible light, therefore the resolution of the electron microscope is better than a light microscope (approx. 2 general types of electron microscopy have been developed: transmission electron.
