01:119:117 Chapter Notes - Chapter 7: Dna Extraction, Ethidium Bromide, Microbiological Culture
7 May 2018
School
Department
Course
Professor
Lab 7 Assignment – Practice Gel Analysis
Name: Zunny Castillo Section: 07
Gel #1: Analyze the gel as a whole using the first gel picture on the next page. Complete the
first column in the table below selecting the best answer only, if applicable, with an “X” to
describe any possible aberrant results affecting the entire gel.
Gel #2, Student #2: Analyze the gel as a whole using the second gel picture on the next
page. Complete the second answer column in the table below selecting the best answer only,
if applicable, with an “X” to describe any possible aberrant results affecting the entire gel.
Analyze the quadrant of the gel for the designated student number. Complete the remaining
columns in the table below. Answer the following questions about the labeled gel. For the
Explanation section, put an “X” in boxes where the option is possible. Leave boxes where the option
is not possible blank.
Gel number
1
2
2
2
2
Sample name
A-COI
P-rbcL
B-16S
Number of PCR bands in the lane
2
1
1
Approximate sizes of the bands in the lane (bp)
700,280
590
1190
Explanation
Best
Best
Possible
Possible
Possible
Ethidium bromide was not added to the gel
Component of the Taq mix was degraded
No primers in the master mix
X
X
X
DNA template not added to PCR tube
Not enough DNA in genomic DNA extraction or
bacterial culture
X
Contamination with PCR product from another
lane
X
Non-specific primer binding
X
Samples switched when loaded on the gel
PCR was performed correctly and this sample
should be sequenced
X
Post this completed document to Sakai under Assignments. Be sure to name your files according
to the instructions on page 4 in Lab 1 (section number - team number - first name last initial –
Practice Gel). It is due before the beginning of your next lab period. Remember that all
assignments should be done individually. When you post your assignment, ensure you get an
email from Sakai that your upload was successful.
find more resources at oneclass.com
find more resources at oneclass.com
Document Summary
Gel #1: analyze the gel as a whole using the first gel picture on the next page. Complete the first column in the table below selecting the best answer only, if applicable, with an x to describe any possible aberrant results affecting the entire gel. Gel #2, student #2: analyze the gel as a whole using the second gel picture on the next page. Complete the second answer column in the table below selecting the best answer only, if applicable, with an x to describe any possible aberrant results affecting the entire gel. Analyze the quadrant of the gel for the designated student number. Complete the remaining columns in the table below. Answer the following questions about the labeled gel. Explanation section, put an x in boxes where the option is possible. Leave boxes where the option is not possible blank. Approximate sizes of the bands in the lane (bp) Ethidium bromide was not added to the gel.
