BIO 310 Chapter Notes - Chapter 12: Nuclear Pore, Nuclear Localization Sequence, Cytosol
Document Summary
Used for transportation of molecules between cytosol and nucleus. Mediated by importins (cytosol nucleus) and exportins (nucleus cytosol) Importins bind to nuclear localization signals (nls) and take specific proteins that need to be transported. Exportins bind to nuclear export sign (nes), which are hydrophobic molecules. Importin binds with cargo protein, ran-gdp, and nls. Nls allows the complex to go through the nuclear pore and into the nucleus. Import receptor has a high affinity towards gtp interacts with ran-gef and switches. Complex has a high affinity for gdp will interact with ran gap to switch gtp for. Nfat is a tf that is in the cytoplasm. Kept in the cytoplasm b/c it"s phosphorylated on threonine and serine residue block nls. Release of ca2+ into the cytoplasm when t-cells are activated. Calmodulin can activate several proteins when in the right shape (influenced by. Calcineurin is activated when it is associated with calmodulin. Activated calcineurin is dephosphorylated and can enter nucleus.
