BIO SCI 98 Chapter Notes - Chapter 9-10: Electron Affinity, Specific Activity, Centrifugation
9 Mar 2018
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Must initially tag the protein first before starting. Eliminating the total protein and part of our unwanted. Specific activity increases as the total grams for protein decreases (more and more purified) Specific activity: ratio of activity of protein: total protein measure how pure your sample is. Cell lysis = break open and extract the cell contents. Fractionation: process of separating the cell compartments. Nucleus will be first because it is the most dense. Depending what protein you want will determine what pellet you want. Highest activity is where you find your protein (if it was secreted you would not find it) Ph values above the pi = protein is negatively charged. Ph values below the pi = protein is positively charged. At the pi solubility is the lowest. Protein activity sensitive to high salt concentrations. Purify individual chemical compounds from mixture of compounds based on their pace from stationary to mobile phase macromolecules from smaller molecules.
