BIO SCI E154 Chapter 1-3: Chapter Summary 1
18 Dec 2016
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Because deoxyribose has no oxygen on the 2" carbon, the molecule is more stable than rna because it is less vulnerable to hydrolysis. It however is important that rna can be easily broken down by hydrolysis because the cell needs to maintain levels of rna. Dna ligase not required but thermostable dna polymerase taq is needed to withstand the denaturation phase. Pyrimidine (one 6 c ring) - n1 atom binds to sugar c1. Purine (2 rings) - n9 binds to sugar c1. Phosphodiester bond --> 3" carbon to 5" carbon of adjacent nucleotide. 2 terminal phosphate released after phosphodiester bond is formed. Helical unwinding, atp hydrolysis supplies energy to break energy bonds to form rep. bubble. Primers provide free 3" oh groups where dna pol adds new nucleotides. The primer is rna created by rna primase and is synthesized by rna pol. Leading strand replicates continuosly towards the opening replication bubble. (5" to 3")
