BIO SCI M125 Chapter 13-15: Chapter Summary 7
21 Dec 2016
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Polymerization (irreversible) (dnmp)n + dntp --> (dnmp)n+1 + ppi. *requires energy because it is losing ppi after using triphosphate. Pyrophophorlysis (dnmp)n + ppi --> (dnmp)n-1 + dntp. *does not require energy, it is adding ppi to chain to remove --> dntp. *loses energy to add on another dnmp base. Reference: parental - its not synthesizing, its unwinding towards the fork. Ssb follows helicase to protect ssdna and prevent it from winding on itself (negative supercoil) 3" to 5" exonuclease to remove base nucleotide. 5" to 3" exonuclease activity - removes rna prrimer and pol 1 replaces with dna nucleotides. Tyr attacks phopshodiester bond, cleaves bond and creastes 5"phosphtyrosyl protein dna linkage. (nucleophilic attack) *no atp is required to restore phosphdiester bond. On lagging strand, once dna pol1 removes rna primer, there is 5" over hang. Elongates longer side and sets complemetary primer --> dna pol 3 synthesize by adding nucleotides to 3" oh end.
