LIFESCI 23L Chapter Notes - Chapter F: Nublu Club, Glycerol, Acrylamide
Document Summary
Estimate protein mass, # of subunits of protein. Rhinovirus - becomes part of your body if you have a cold. Myoglobin, hemoglobin = small chaperon, ribosome = large protein. Secondary - regular repeated patterns, uses h bonding. Bpti - 3 disulfide bridges, if sulfide bridges are removed it unfolds at room temp. Carbon alpha backbone trace, ball and stick, ribbons, solvent- accessible surface. Purify single protein from all other cell components. Proteins travel until their density matches the surrounding sucrose. Proteins can be separated by size, hydrophobicity, charge, binding to specific groups. Gel electrophoresis separate a mixture of proteins based on size. Electric field applied so migrates to postive side. Might not separate by size but rather charge/shape. Use bis tris methane instead of tris-glycerine with lower ph. Lower ph allows acrylamide gel to be more stable, longer storage. Gel w/ proteins blotted to more durable membrane. Mem brane treated w/ antibody specific to protein of interest.
