LIFESCI 3 Chapter Notes - Chapter 18.3: Kozak Consensus Sequence, Chloroplast, Helicase
Document Summary
Recruitment of small ribosomal unit to mrna. Association of the charged trna with the mrna. Recruitment of the large ribosomal subunit to form an active ribosome. Initiation factors: enhance the rate and fidelity of translation initiation. Fine tune rrna-based activities and interactions among mrna, trna, and rrna. Base pairing recruits the small ribosomal subunit to bacterial mrnas. Shine-dalgarno sequence (ribosome binding site): guides the initiation codon in bacteria to the correct position on the ribosome. Base pairs with a pyrimidine rich sequence near the 3" end of the 16s rrna of the 30s ribosomal subunit. Puts start codon in the precise location on the 30s subunit for initiation. Higher degree of base pairing complementarity and optimal spacing between the sequence and the initiation codon increased efficiency of translation of a particular mrna. Start codon aug is distinguished from other aug codons by proximity to the shine-
