ANSC 3323 Chapter Notes - Chapter 2: In Vitro Maturation, Embryo Transfer, Controlled Ovarian Hyperstimulation

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30 Sep 2016
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Steps of generating embryo outside the body (in vitro) Ivp/ivf & opu: superovulation, oocyte retrieval (sperm collection, in vitro maturation (ivm, in vitro fertilization (ivf, in vitro production (ivp, in vitro culture (ivc, genetic testing, embryo-transfer (et, performed in humans and cattle. Ovum pick-up (opu: allows elite animals to reproduce more, oocytes retrieved used to produce embryos, no pregnancy for oocyte donors, track pedigree ivp, opu in cattle. Collection of oocytes from ovaries by ultrasound-guided needles and aspiration. Oocytes are then used for embryo production. Red, raised, bulbous areas are follicles (have one oocyte each) Opu can be conducted every 3 days. Follicular development in cattle: cohort (a bunch of follicles that develop at the same time) Recruitment (cohort development; many follicles are recruited) Atresia (ovulate dominant follicle: first appear by 90 days" gestation, females are born with follicles already formed, follicles develop in waves (2-3 waves) Pregnant cows: cattle natural lifespan = 10-11 years.

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