ANSC 3323 Chapter Notes - Chapter 5: Intracytoplasmic Sperm Injection, Abdominal Ultrasonography, Transvaginal Oocyte Retrieval
Document Summary
Egg retrieval: done under iv sedation provided by a certified anesthesiologist, ultrasound is used to retrieve the eggs (as in opu, risks of egg retrieval. Sperm in ivf: sperm sample produced at time of egg retrieval, consider freezing backups before, insemination is done the afternoon of egg retrieval, sperm donation. Offspring are healthier and more fertile in response. Most human ivf clinics use isci for fertilization: sperm injection variations. Isci (intracytoplasmic sperm injection: the majority of ivf cycles are icsi cycles. Embryo transfer: most transfers at day 3. Easier to maintain outside of culture than the blastocyst. Day 5 blastocyst stage embryo in humans. Woman"s age is the most important factor. Acceptability of multi-fetal/selective reduction of # of fetuses. Couple"s feeling with regards to selective reduction of fetuses. Day 3 (8 cell) vs. day 5 (blastocyst) Additional procedure (done in conjunction w/ human ivf) Assisted hatching: an opening/thinning is created in the zona pellucida to help embryo to hatch.
