MCB 2610 Chapter Notes - Chapter 6B: Protein Structure, Pyrimidine Dimer, Geobacillus
8 Sep 2016
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Avery welch mcb 2610 microbiology week 6-7 chapter 6b eliminating microbes: moist heat. Boiling will not destroy spores and does not sterilize. Degrades nucleic acids, denatures proteins, and disrupts membranes. Boiling is not a sterilization method because it does not kill endospores. Must be carried out above 100 degrees celsius which requires saturated steam under pressure. Spores are killed by combination of heat and pressure as well as mostly everything else. If pressure is not working properly, spores will not be killed by heat alone. Carried out using an autoclave (developed by chamberland, 1884) 121 c at 15 psi for 10-15 min. Effective against all types of microorganisms including spores. Quality control includes strips with geobacillus stearothermophilus. Can put tubes and plates in an autoclave, but not plastic (that is not heat safe) You cannot use the autoclave for antibiotics & vitamins because they need to maintain their structure, oils, and waxes.
