ANTHRBIO 201 Chapter Notes - Chapter 11: Transcription Factor Ii B, Covalent Bond, Thymidine

• School: U of M
• Department: Anthropology, Biological
• Course: ANTHRBIO 201
• Professor: John Mitani

Document Summary

General transcription factors (bind to core promoter elements, gene-specific transcription factors (bind to promoter proximal and enhancer elements). General transcription factors and assembly of preinitiation complex. Figure 11 shows the sequential building the the factors and polii to form the preinitiation complex. One or more purified factors were bound to radiolabeled adenovirus promoter dna. The band at the bottom is the input dna. Fig 11. 1a: without iid, no complex (iid is critical, no iib you get da. Reiterates that da can bind without b: no iia, you get dbpolf (iia is not that critical for this complex to assemble on this dna, iie and iih bind onto the dbpolf complex. What about iia: the location that d, da and dab bound were determined by footprinting (fig 11. 2). They did footprints with either the template or nontemplate strands labeled. Dnase and a new method using a 1,10 phenanthroline(op) copper ion complex.