BIOL 257 Chapter Notes - Chapter 3: Refraction, Antibody, Virtual Image
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3. 1 methods of culturi(cid:374)g microorga(cid:374)is(cid:373)s: the five i"s. The five i"s: 1. ) inoculation: to cultivate, or culture, microorganisms, one introduces a tiny sample into a container of nutrient medium, which provides an environment in which they multiply. Any instrument used for sampling and inoculation must initially be sterile: 2. ) Incubation: after inoculation, the medium is placed in a temperature- controlled chamber to encourage multiplication. Usual temperature is b/t 20 and 45-degree c. Chemical composition functional type(purpose: types of media: Physical state: liquid: water-based solutions that do not solidify at temperatures above freezing and that tend to flow freely. Semisolid media: exhibit a clot like consistency because thy co contain an amount of solidifying agent that thickens them but does not produce a firm surface. Solid media provide a firm surface on which cells can form discrete colonies and are advantageous for isolating and culturing bacteria and fungi.
